materials antibodies Search Results


s19 compound pa463  (Chem Impex International)
95
Chem Impex International s19 compound pa463
S19 Compound Pa463, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s19 compound pa463/product/Chem Impex International
Average 95 stars, based on 1 article reviews
s19 compound pa463 - by Bioz Stars, 2026-03
95/100 stars
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pcm1  (Proteintech)
93
Proteintech pcm1
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Pcm1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcm1/product/Proteintech
Average 93 stars, based on 1 article reviews
pcm1 - by Bioz Stars, 2026-03
93/100 stars
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tris  (Chem Impex International)
96
Chem Impex International tris
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Tris, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tris/product/Chem Impex International
Average 96 stars, based on 1 article reviews
tris - by Bioz Stars, 2026-03
96/100 stars
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fak antibody  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc fak antibody
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Fak Antibody, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak antibody/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
fak antibody - by Bioz Stars, 2026-03
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mouse anti-c-myc-tag antibody  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc mouse anti-c-myc-tag antibody
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Mouse Anti C Myc Tag Antibody, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-c-myc-tag antibody/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
mouse anti-c-myc-tag antibody - by Bioz Stars, 2026-03
90/100 stars
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reference materials for mpo-anca antibodies  (International Federation of Clinical Chemistry and Laboratory Medicine)
90
International Federation of Clinical Chemistry and Laboratory Medicine reference materials for mpo-anca antibodies
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Reference Materials For Mpo Anca Antibodies, supplied by International Federation of Clinical Chemistry and Laboratory Medicine, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reference materials for mpo-anca antibodies/product/International Federation of Clinical Chemistry and Laboratory Medicine
Average 90 stars, based on 1 article reviews
reference materials for mpo-anca antibodies - by Bioz Stars, 2026-03
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α-his antibody  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc α-his antibody
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
α His Antibody, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/α-his antibody/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
α-his antibody - by Bioz Stars, 2026-03
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polyclonal rabbit anti-mbp  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc polyclonal rabbit anti-mbp
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Polyclonal Rabbit Anti Mbp, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal rabbit anti-mbp/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-mbp - by Bioz Stars, 2026-03
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anti-β-actin mouse monoclonal antibody  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc anti-β-actin mouse monoclonal antibody
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Anti β Actin Mouse Monoclonal Antibody, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-β-actin mouse monoclonal antibody/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
anti-β-actin mouse monoclonal antibody - by Bioz Stars, 2026-03
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anti-actin mouse monoclonal antibody  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc anti-actin mouse monoclonal antibody
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Anti Actin Mouse Monoclonal Antibody, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-actin mouse monoclonal antibody/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
anti-actin mouse monoclonal antibody - by Bioz Stars, 2026-03
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antibody y051377  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc antibody y051377
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Antibody Y051377, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody y051377/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
antibody y051377 - by Bioz Stars, 2026-03
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mouse antibody against βtubulin  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc mouse antibody against βtubulin
Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, <t>PCM1,</t> and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.
Mouse Antibody Against βtubulin, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse antibody against βtubulin/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
mouse antibody against βtubulin - by Bioz Stars, 2026-03
90/100 stars
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Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, PCM1, and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.

Journal: Cell reports

Article Title: CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

doi: 10.1016/j.celrep.2020.107630

Figure Lengend Snippet: Figure 6. CCDC57 Is Required for Efficient Ciliogenesis and Centriolar Satellite Distribution (A) Effect of CCDC57 loss on cilium formation. Control and CCDC57-depleted RPE1 cells were serum-starved for 48 h, and percentage of ciliated cells was determined by staining for acetylated-tubulin, glutamylated tubulin, PCM1, and DAPI. (B) Quantification of ciliogenesis experiments. Results shown are the mean of three independent experiments ± SEM (>300 cells per experiment; **p < 0.01). (C) Effect of CCDC57 depletion on cilium length. Cilium length was determined by staining for acetylated-tubulin and DAPI. Results shown are the mean of three independent experiments ± SEM (>100 cells per experiment; ****p < 0.0001). (D) mNeonGreen (mNG)-CCDC57 (1–502) stable expression rescues the ciliogenesis defect of CCDC57-depleted cells. U2OS cells stably expressing mNG, mNG-CCDC57 (1–502), or mNG-CCDC57 (503–916) were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and 24 h serum starvation and were stained for cilium markers. Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; *p < 0.05; n.s, non-significant). (E) Effect of CCDC57 depletion on centriolar satellite distribution. U2OS cells were fixed 86 h after transfection with control siRNA or CCDC57 siRNA and stained for the indicated proteins. Images represent centrosomes in cells from the same coverslip taken with the same camera settings. (F) Quantification of (E). PCM1 and CEP131 fluorescence intensities at the centrosome were measured from SUM projections, and average means of the levels in control cells were normalized to 1. Data represent mean values from two experiments ± SEM (>50 cells per experiment; **p < 0.01; ****p < 0.0001). (G) Effects of PCM1 depletion on ciliogenesis efficiency of CCDC57-depleted cells. RPE1 cells were transfected with control siRNA, CCDC57 siRNA, or CCDC57/ PCM1 siRNAs for 86 h; serum-starved for the last 24 h; fixed; and stained for cilium markers. (H) Quantification of (G). Results shown are the mean of two independent experiments ± SEM (>100 cells per experiment; **p < 0.01; ***p < 0.001). Scale bars, 10 mm.

Article Snippet: Primary antibodies used for immunoblotting were rabbit anti PCM1 (Proteintech, 19856-1-AP) at 1:500, rabbit anti-beta-actin (Cell Signaling Technology) at 1:10000, mouse anti alpha-tubulin (Sigma, DM1A) at 1:5000, anti-CCDC57 (Sigma HPA023344) at 1:1000, anti-c-Myc (clone 9E10) at 1:500, rabbit anticapsase-3 (Proteintech) at 1:500 and mouse anti-acetylated tubulin (clone 6-11B, 32270, Thermo Fischer) at 1:5000. anti-PCM1 and anti-GFP antibodies were generated and used for immunobloting as previously described (Firat-Karalar et al., 2014).

Techniques: Control, Staining, Expressing, Stable Transfection, Transfection